Journal: Journal of Translational Medicine

Article Title: Limonin attenuates hyperlipidemia by regulating the gut microbiota-bile acid-farnesoid X receptor axis

doi: 10.1186/s12967-026-07826-7

Figure Lengend Snippet: Impacts of Limonin upon FXR/FGF15 signaling pathway in intestinal of hyperlipidemia mice. ( A ) Representative picture of immunofluorescence expression in mice intestine. ( B ) The immunofluorescence protein expression of FGF15 in the ileum. ( C ) The immunofluorescence protein expression of FXR in the ileum. ( D ) Ileum mRNA expression levels of Fgf15 . ( E ) Ileum mRNA expression levels of Fxr . ( F ) Ileum mRNA expression levels of Asbt . ( G ) Representative protein bands detected by WB. ( H ) Ileum protein expression levels of FGF15. ( I ) Ileum protein expression levels of FXR. ( J ) Ileum protein expression levels of ASBT. Values are shown as mean ± SD ( n = 3). # P < 0.05, ## P < 0.01 vs. MG and * P < 0.05, ** P < 0.01 vs. NG

Article Snippet: The protein expression of FXR (BOSTER, M03308), FGF15 (Cohesion, CQA5895), ASBT (Cohesion, CQA5895), and FGFR4 (Cohesion, CPA9200) in the liver and ileum were detected by TSA multi-marker staining technique.

Techniques: Immunofluorescence, Expressing

Journal: Journal of Translational Medicine

Article Title: Limonin attenuates hyperlipidemia by regulating the gut microbiota-bile acid-farnesoid X receptor axis

doi: 10.1186/s12967-026-07826-7

Figure Lengend Snippet: Impacts of Limonin upon FXR/FGF15 signaling pathway in hyperlipidemia mice liver. ( A ) mRNA expression levels of fgf15 in liver. ( B ) mRNA expression levels of Fgfr4 in liver. ( C ) mRNA expression levels of Cyp7a1 in liver. ( D ) Representative picture of immunofluorescence expression in mice liver. ( E ) F Immunofluorescence expression of CYP7A1 in liver. ( F ) Immunofluorescence expression of CYP7A1 in liver. ( G ) Immunofluorescence expression of CYP7A1 in liver. ( H ) FGF15 protein expression levels. ( I ) FGFR4 protein expression levels. ( J ) CYP7A1 protein expression levels. ( K ) Representative protein bands detected by WB. Values are denoted by mean ± SD ( n = 3). # P < 0.05, ## P < 0.01 vs. MG and * P < 0.05, ** P < 0.01 vs. NG

Article Snippet: The protein expression of FXR (BOSTER, M03308), FGF15 (Cohesion, CQA5895), ASBT (Cohesion, CQA5895), and FGFR4 (Cohesion, CPA9200) in the liver and ileum were detected by TSA multi-marker staining technique.

Techniques: Expressing, Immunofluorescence

Journal: Journal of Translational Medicine

Article Title: Limonin attenuates hyperlipidemia by regulating the gut microbiota-bile acid-farnesoid X receptor axis

doi: 10.1186/s12967-026-07826-7

Figure Lengend Snippet: Graphical illustration of mechanism mediating anti-hyperlipidemia effects of Limonin. The possible mechanisms by which limonin alleviates dyslipidemia are as follows: (1) Reduced relative abundance of BSH-producing microbes induced by Limonin. (2) Boosted conjugated BAs, particularly TCA, T-αMCA, and T-βMCA acting in antagonistic manner on intestinal FXR, (3) The increased conjugated BAs boosted elimination of BAs via feces, and also as endogenous signaling molecules that inhibited activation of intestinal FXR causing FGF15’s reduced production in the distal ileum. (4) The FGF15 through the enterohepatic circulation to enter mice liver, and regulates liver CYP7A1 expression via negative feedback of FGF15-FGFR4 pathway to promoting the synthesis of TC to BA, thereby reducing TC

Article Snippet: The protein expression of FXR (BOSTER, M03308), FGF15 (Cohesion, CQA5895), ASBT (Cohesion, CQA5895), and FGFR4 (Cohesion, CPA9200) in the liver and ileum were detected by TSA multi-marker staining technique.

Techniques: Activation Assay, Expressing

Journal: PeerJ

Article Title: Dieting alleviates hyperuricemia and organ injuries in uricase-deficient rats via down-regulating cell cycle pathway

doi: 10.7717/peerj.15999

Figure Lengend Snippet: (A) Casp3, Cdk1 and Ki67 in the liver was evaluated at mRNA level, and a high value of FPKM means a high expression; (B) Casp3, Cdk1 and Ki67 in the liver was evaluated by Western blot; C, Results of B were quantified by relative brightness. FPKM, Fragments Per Kilobase of exon model per Million mapped fragments. Control, control group, KDY rats were given free approach to food and water; 75% dieting, 75% dieting group, rats were given 75% food of control group at the same age in days and given free access to water. * P < 0.05 vs control group, Student’s t -test, two-tailed.

Article Snippet: Hematoxylin-eosin (HE) staining kits, mouse anti- β -actin antibody (BM0627), rabbit anti-CASP3 antibody (BA2142), rabbit anti-CDK1 antibody (PB9533), rabbit anit-Ki67 antibody (M00254-4), and horseradish peroxidase (HRP) conjugated goat anti-rabbit/mouse IgG (H+L) (BA1056) were all purchased from Boster Biological Engineering Co., Ltd (Wuhan, China).

Techniques: Expressing, Western Blot, Control, Two Tailed Test

Journal: Genes & development

Article Title: Distinct molecular mechanisms regulate cell cycle timing at successive stages of Drosophila embryogenesis

doi:

Figure Lengend Snippet: Summary, showing fluctuations in levels of String (bold line), Cyclin B (dashed line), and Cdc2 (fine line) during the first 14 cell cycles. Protein levels are depicted along the y-axis on a linear scale, with arbitrary units. Time is indicated along the x-axis, with periods of mitosis boxed in black and numbered. During cycles 1–7, there is little fluctuation in Cyclin levels or Cdc2 kinase activity. Beginning at cycle 8, Cyclin degradation at metaphase/anaphase transitions becomes apparent, and the cycles begin to slow. At this point Cdc2 kinase activity also begins to fluctuate, due to gain and loss of the activating phosphate at T161. It is note-worthy that greater amounts of Cyclin are degraded with progressively later cycles. Because nuclei and mitotic apparatuses (presumed Cdc2/Cyclin substrates) increase exponentially in number, this suggests a connection between Cyclin utilization and its degradation. Accordingly, we propose that cycle slowdown following mitosis 8 is effected by titration and depletion of maternal Cyclins (Table 1). Mitosis 14 is timed by a distinct mechanism, in which inactive Cdc2/Cyclin complexes are activated by String translated from new zygotic transcripts.

Article Snippet: Zygotic cell cycle control begins during interphase 14, and a series of slower, spatially patterned, G 2 -regulated cell cycles follow ( Hartenstein and Campos-Ortega 1985 ; Foe 1989 ; Edgar and O’Farrell 1990 ).

Techniques: Activity Assay, Titration